- Introduction to single-cell RNA-seq
- Quality control and normalization
- Survey of downstream analysis methodology
- Full-length transcript vs 3’ end only
- Plate-based vs droplet-based
- Read counts vs UMIs
- Multiplexed with barcodes vs not
July 6, 2019
Bulk RNA-seq measures the average expression across a pool of cells
Hicks et al. 2018 (https://doi.org/10.1093/biostatistics/kxx053)
Isoform analysis only possible with full-length
image source: http://data-science-sequencing.github.io/Win2018/lectures/lecture16/
UMIs + Barcodes can be added with specially designed plastic or hydrogel beads
Macosko et al. 2015 (https://doi.org/10.1016/j.cell.2015.05.002)
Ziegenhain et al. 2017 (https://doi.org/10.1016/j.molcel.2017.01.023)
da Veiga Beltrame et al. 2019 (https://doi.org/10.6084/m9.figshare.7704659.v1)